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Pf1 Filamentous Phage

NMR co-solvent for residual dipolar coupling (RDC) measurements

Reliable RDC data can be best obtained by using filamentous bacteriophages such as the Pseudomonas aeruginosa phage Pf1 in RDC experiments.
Hyglos GmbH specializes in the use of bacteriophages in biotechnology. Our outstanding expertise in propagation, purification and handling of bacteriophages enables us to produce RNase and protease free Pf1 phage of superior quality.

PF1 Filamentous Phage

Pf1 50 mg Protease & RNase free

Item no. 311079
50 mg, other sizes on request

Advantages of residual dipolar coupling (RDC):

  • structural quality indicator (structure validation)

  • can be used for automatic NMR assignments (MARS)

  • can be used to distinguish oligomeric states

  • clearly identifies which structure obtained by crystallography is representative of the solution conformation

  • complements intramolecular NOE (Nuclear Overhauser Effect) constraints for backbone fold

  • indicates the quality of the relative orientation of the subunits within a complex

  • provides an accurate and simple means of identifying regions in a protein crystal structure with conformation significantly altered by crystal packing

  • provides precise long-range orientation constraints

  • replaces many intermolecular NOEs traditionally needed to dock complexes

Advantages of filamentous phages in dipolar coupling experiments:

  • aligns stably in a wide temperature range

  • degree of alignment can be easily tuned by changing the phage concentration

  • easy to separate from the macromolecule of interest by ultracentrifugation

  • extremely stable under physiological conditions

  • no effect on the rotational correlation time of nucleic acids

  • phage macromolecule system is stable (for a long shelf life)