EndoTrap® HD - High-Definition Endotoxin Removal

New Performance Data for EndoTrap® HD 

A new study describes the efficiency of four endotoxin removal resins from different suppliers including EndoTrap®. EndoTrap® HD supplied by Hyglos was shown to be the most effective resin for removing endotoxins in comparison to the other resins tested.

EndoTrap® HD - High-Definition Endotoxin Removal

EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.

The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates.
EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, Hyglos' High-Definition Matrix is also available for small-scale purification as pre-filled columns.

Endotoxin removal efficiency of EndoTrap® HD:

SampleSample conc.
[mg/ml]
Initial LPS level
[EU/ml]
LPS level after EndoTrap® HD
[EU/ml]
LPS removal efficiency
[%]
Sample recovery
[%]
BSA102687.60 < 0.15 > 99.9998.0
MAK  11004.95 < 0.15> 99.9995.1
Phage-protein 11322.15< 0.15> 99.99100.0
POD1750.27 < 0.15> 99.99100.0

EndoTrap® HD features:

  • Best-in-class endotoxin binding capacity of > 5,000,000 EU/ml
  • Removes more than 99.99% of LPS
  • Excellent sample recovery rate >95%
  • Non-toxic bacteriophage-derived ligand, no cytotoxic effects
  • Re-use more than 10 times
  • pH sample range: 4-10
  • suitable for high salt conditions
  • suitable for e.g. TRIS, HEPES, MOPS or similar buffered samples
  • PBS buffer possible when enriched freshly with 0.1 mM calcium (magnesium) directly before use
  • EndoTrap® Leakage ELISA for ligand leakage monitoring
  • Regulatory Support File (EndoTrap® HD) available on request

Available as resin (buffers on request).

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Product informations

Publications and references

EndoTrap HD 1/1 Endotoxin Removal Column binds 5,000,000 EU/mL.  Re-use: 10x

EndoTrap® HD 1/1

Item no. 800053
1 x 1 ml Column, 125 ml Equilibration Buffer, 125 ml Regeneration Buffer,
125 ml Storage Buffer
Price (page 2)


EndoTrap® HD 5/1 Endotoxin Removal Columns bind 5,000,000 EU/mL.  Re-use: 10x

EndoTrap® HD 5/1

Item no. 800063
5 x 1 ml Column, 125 ml 5x Equilibration Buffer, 125 ml 5x Regeneration Buffer,
125 ml 5x Storage Buffer
Price (page 2)


EndoTrap HD 10 Endotoxin Removal Kit binds 5,000,000 EU/mL.  Re-use: 10x

EndoTrap® HD 10

Item no. 800034
10 ml settled resin
Price (page 2)


EndoTrap HD 50 Endotoxin Removal Kit binds 5,000,000 EU/mL. 10x regenerable

EndoTrap® HD 50

Item no. 800035
50 ml settled resin
Price (page 2)


EndoTrap HD 250 Endotoxin Removal Kit binds 5,000,000 EU/mL. 10x regenerable

EndoTrap® HD 250

Item no. 800036
250 ml settled resin
Price (page 2)


EndoTrap HD bulk Endotoxin Removal resin binds 5,000,000 EU/mL. 10x regenerable

EndoTrap® HD Bulk

Item no. 800037
Bulk resin
price and availability on request


EndoTrap Leakage Elisa for endotoxin (LPS) binding ligand determination

EndoTrap® Leakage ELISA for EndoTrap HD/blue

Item no. 800033
12 x 8 well stripes, 1 vial POD-Antibody,
1 vial Standard, 20 ml ABTS Substrate, ready to use                      
Price (page 2)