EndoTrap® family: Endotoxin removal products
EndoTrap®, affinity chromatography for highly efficient endotoxin removal.
EndoTrap® reliably removes endotoxins (lipopolysaccharides / LPS) from aqueous solutions of e.g. proteins, antibodies, vaccines, nucleic acids, buffers and various other substances with excellent sample recovery rates.
EndoTrap® features:
Highly specific and efficient LPS removal from complex mixtures
Quick and easy to use in column (gravity flow) or batch mode
Upscaling on fully automated liquid-chromatographic systems possible
Average sample/protein recovery > 95%
Broad pH tolerance and broad pI range of target proteins
Compatible with commonly used buffers and high salt conditions
Reusable at least 10 times (EndoTrap® HD)
Not Polymyxin B based, non toxic
Regulatory Support File (EndoTrap® HD) and toxicological data available on request
The EndoTrap® product family consists of three members
(see "Short Introduction EndoTrap®-family" in service box):
EndoTrap® accessory equipment:
Endotoxin removal efficiency from various samples with EndoTrap® HD
| Sample | pl | Sample Volume (ml) | Sample conc. (mg/ml) | EU before polishing (EU/ml) | EU after polishing (EU/ml) | LPS removal efficiency (%) | Sample recovery (%) | Ligand leakage (ng/ml) |
|---|---|---|---|---|---|---|---|---|
| BSA | 4.7 | 1000 | 1 | 577 | 0.027 | > 99.99 | 96 | 0.573 |
| lgG | 8.3-8.9 | 1000 | 1 | 1317 | < 0.025 | > 99.99 | 98 | 0.334 |
| Lysozyme | 10.7 | 1000 | 1 | 255 | < 0.025 | > 99.99 | 95 | 1.40 |