EndoZyme® Recombinant Factor C (rFC) Assay
EndoZyme® is intended for in vitro quantitative determination of endotoxin (lipopolysaccharide, LPS) in pharmaceuticals and biological substances as well as for medical device testing.
The endotoxin-specificity of EndoZyme® eliminates all false-positive and elevated results seen with LAL due to ß-glucan reactions in the LAL cascade. Adding the ease-of-use and excellent lot-to-lot consistency, EndoZyme® is a representative of modern recombinant methods, moving away from the animal source which for decades has been the horseshoe crab.
Having a homogeneous assay format without a solid phase, EndoZyme® is best suited for non-complex samples and buffer conditions. For complex sample matrices, EndoLISA® is recommended.
All reagents needed for running EndoZyme® are included in the kit:
EndoZyme®, Item no. 609050 (192 tests)
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Included: Enzyme Recombinant Factor C, Substrate, Endotoxin Standard, Water (endotoxin-free), Assay Buffer, Assay Plates (2 x 96 wells).
Specifications and advantages:
- Highly sensitive recombinant Factor C endotoxin detection assay, measurement range 0.005-50 EU/ml
- No false-positive glucan reaction due to endotoxin-specific recombinant technology
- Excellent reliability and lot-to-lot consistency
- All needed reagents included in the kit
- No animal material, therefore saving the diminishing horseshoe crab population
- For laboratory and research use only
Additional material needed:
- Fluorescence microplate reader
- Lab shaker
- Endotoxin-free disposables and glassware