EndoZyme® recombinant Factor C (rFC) Assay

EndoZyme® is an endpoint fluorescent microplate assay intended for in vitro quantitative determination of endotoxin (lipopolysaccharides, LPS) in pharmaceuticals and biological substances as well as for medical device testing.

The endotoxin-specificity of EndoZyme® eliminates all false-positive and elevated results seen ß-glucan reactions in the LAL test. Adding the ease-of-use and excellent lot-to-lot consistency, the LAL alternative EndoZyme® is a representative of modern recombinant methods, moving away from the animal source which for decades has been the horseshoe crab.

Being a homogeneous assay without solid phase, EndoZyme® is particularly suited for non-complex samples and buffer conditions. For complex sample matrices, EndoLISA® is recommended.

All reagents needed for running EndoZyme® are included in the kit:

EndoZyme Recombinant Factor C Endotoxin Detection Assay

EndoZyme®, Item no. 609050 (192 tests)

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Included: Enzyme (recombinant Factor C), Substrate, Endotoxin Standard, Water (endotoxin-free), Assay Buffer, Assay Plates (2 x 96 wells).

Specifications and advantages:

  • Highly sensitive rFC endotoxin detection assay with measurement range 0.005 - 50 EU/ml
  • No false-positive glucan reaction due to endotoxin-specific recombinant technology
  • Excellent reliability an dlot-to-lot reproducibility
  • All needed reagents included in the kit
  • Flexible testing format: microtiter plate
  • No animal material, therefore saving the diminishing horseshoe crab population
  • For laboratory and research use only

Additional material needed:

  • Fluorescence microplate reader
  • Lab shaker/vortex mixer
  • Endotoxin-free disposables and glassware

References:

EndoZyme® has been featured in diverse publications and has several reference users.