EndoLISA® ELISA-based Endotoxin Detection Assay

EndoLISA® is an endpoint fluorescent microplate assay intended for in vitro quantitative determination of lipopolysaccharide (LPS) in pharmaceutical, biological and environmental samples.

Revolutionizing endotoxin testing with built-in sample preparation, EndoLISA® is the result of several years of research and test development aimed at establishing a better performing alternative to LAL. In the recently published book "Pharmaceutical Microbiology" (Wiley-VCH), Dr. Michael Rieth describes EndoLISA® as a milestone in endotoxin detection.

EndoLISA® is based on a lipopolysaccharide (LPS) specific phage recombinant protein, which specifically binds to the entire substance group of LPS (endotoxin). The phage protein is pre-coated to the wells in the EndoLISA® microtiter plate and as the sample is added to the well, the endotoxin (LPS) in the sample is bound to the phage protein. Any sample matrix with potentially interfering components is then completely removed by a washing step. Therefore, the subsequent detection by recombinant Factor C (rFC) and a fluorescence substrate is left unaffected by inhibitors, facilitating a reliable quantification of endotoxin in the sample.

All reagents needed for running EndoLISA® are included in the kit:

EndoLISA Endotoxin Detection Test

EndoLISA®, Item no. 609033 (192 tests)

>> Order

Included: EndoLISA® Plates (2x 96 wells), Water (endotoxin-free), Binding Buffer, Endotoxin Standard, Wash Buffer, Enzyme recombinant Factor C, Substrate, Assay Buffer, Cover Foil.

Specifications and advantages:

  • Overcomes limitations of existing methods, such as the need for substantial dilution
  • Reduced matrix effects due to integrated wash step
  • No interference in high salt conditions e.g. NaCl, GdnHCl
  • Robust assay with excellent reproducibility
  • Measurement range: 0.05 - 500 EU/ml
  • pH range: 4 - 9
  • Flexible testing format: microtiter plate
  • Saves the diminishing horseshoe crab population
  • For laboratory and research use only

Additional material needed:

  • Fluorescence Microplate Reader
  • Lab shaker/vortexer
  • Incubator 37°C
  • Endotoxin-free disposables and glassware